Rinsho Shinkeigaku (Clinical Neurology)

Symposium 06

Identification of NMDA receptor in normal bovine ovary and ovum

Naoko Tachibana, M.D., Ph.D.1) and Shu-ichi Ikeda, M.D., Ph.D.2)

1)Department of Neurology, Okaya City Hospital
2)Department of Medicine (Neurology & Rheumatology), Shinshu University School of Medicine

To clarify the pathogenesis of anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis in patients without ovarian teratoma, we investigate normal human ovary, normal bovine ovary and bovine ova. On the basis of immunohistochemical studies, normal human ovary expressed NR2B epitope in primordial oocytes. The results of SDS-PAGE and immunoblotting using bovine ovarian tissues and ova, we identified two bands of NR1 and NR2B. Moreover, reverse phase liquid chromatography coupled to tandem mass spectrometry showed peptides fractions of NR1, NR2A, NR2B and NR2C. Immunocytochemical study disclosed that normal bovine oocyte has a strong affinity for a patient's disease-specific IgG. Anti-NMDAR encephalitis involves mainly young women who are in their reproductive age. Ovarian teratoma is important as simultaneous tumor, the percentage of patients with ovarian teratoma is less than 40%. It is obvious that the origin of ovarian teratoma is oocyte. So the existence of NMDAR in normal oocytes is very important to assert that ovary itself is the antigen presenting tissue. And also it is helpful to explain why young women are mainly affected from this disease. It seems to conclude that anti-NMDAR encephalitis is one form of autoimmune synaptic encephalitis and that the antigen presenting tissue is ovary itself.
Full Text of this Article in Japanese PDF (239K)

(CLINICA NEUROL, 54: 1031|1033, 2014)
key words: limbic encephalitis, glutamate receptor, N-methyl-D-aspartate receptor, oocyte; ovarian teratoma

(Received: 21-May-14)