臨床神経学

<シンポジウム(1)―4―2>ALSに対する再生医療の開発

幹細胞生物学を応用した神経疾患病態研究

赤松 和土

慶應義塾大学医学部生理学〔〒160―8582 東京都新宿区信濃町35〕

The rapid and efficient induction of neural stem cells (NSCs) from pluripotent stem cells is required for the research of patient-specific iPS cells and regenerative medicine to induce their own neural cells. Here, we induced NSCs from human pluripotent stem cells within 2 weeks and these clonal NSCs were expanded efficiently by their self-renewal ability. Further, we directly induced NSCs from both mouse and human fibroblasts using four reprogramming factors (Oct4, Sox2, Klf4, and cMyc) without the clonal isolation of induced pluripotent stem cells (iPSCs). Since these NSCs rapidly developed into mature gliogenic neural stem cells, we were able to purify these rapidly differentiating NSCs without contamination of differentiation-resistant pluripotent cells. These methods will facilitate high throughput screening of phonotypes appeared in neural cells induced from the somatic cells derived from sporadic neurodegenerative diseases.
Full Text of this Article in Japanese PDF (200K)

(臨床神経, 52:937−938, 2012)
key words:直接誘導,iPS細胞,ES細胞,神経幹細胞

(受付日:2012年5月23日)